Protoplast culture and plant regeneration of an important medicinal plant Tylophora indica were achieved through callus regeneration. Protoplasts were isolated from leaf mesophyll cells and cultured ...

During the isolation of rose petal protoplasts, two successive transient abscisic acid (ABA) accumulations were detected when digest medium and tissue slices were assayed simultaneously, using ...

Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by sequencing, ...

Biotechnological innovation meets horticulture as researchers unveil a cutting-edge genome editing protocol for grapes. This novel technique, integrating protoplast culture with CRISPR-Cas9, ...