Protein Quantification using BCA method
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YouTubeAbhishek Vats
Protein Quantification using BCA method
The Bicinchoninic acid (BCA) protein assay is a colorimetric detection and quantification of total protein. This is based on the reduction of Cu+2 to Cu+1 by protein peptide bond and amino acid in alkaline medium known as the biuret reaction. Further, the chelation of two molecules of BCA with one Cu+1 (cuprous ion) gives water soluble complex ...
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